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Immunology and Infection

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1차 인간 단핵구의 고립, 형질전환 및 문화

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation, Transfection, and Culture of Primary Human Monocytes

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1차 인간 단핵구의 고립, 형질전환 및 문화

12,718 Views

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09:13 min

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December 16, 2019

DOI:

10.3791/59967-v

DOI:

10.3791/59967-v

•

09:13 min

December 16, 2019

•

17 Views

Karlie Plaisance-Bonstaff^1,2, Celeste Faia², Dorota Wyczechowska¹, Duane Jeansonne¹, Cecilia Vittori^1,3, Francesca Peruzzi^1,2

¹Department of Medicine,Louisiana State University Health Sciences Center, ²Department of Microbiology, Immunology, and Parasitology,Louisiana State University Health Sciences Center, ³Department of Biomedical and Clinical Sciences L. Sacco,University of Milan

Summary

여기에 제시된 것은 HIV에 감염된 개인과 건강한 대조군으로부터 인간의 1차 단핵구를 분리, 배양, 트랜스펙팅 및 분화하기 위한 최적화된 프로토콜입니다.

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