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Isolation, Transfection, and Culture of Primary Human Monocytes
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JoVE 杂志 免疫与感染
Isolation, Transfection, and Culture of Primary Human Monocytes

Isolation, Transfection, and Culture of Primary Human Monocytes

12,771 Views

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09:13 min

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December 16, 2019

DOI:

10.3791/59967-v

DOI:
10.3791/59967-v

•

09:13 min
December 16, 2019

•

17 Views
, , , , ,
1Department of Medicine,Louisiana State University Health Sciences Center, 2Department of Microbiology, Immunology, and Parasitology,Louisiana State University Health Sciences Center, 3Department of Biomedical and Clinical Sciences L. Sacco,University of Milan

Summary

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Presented here is an optimized protocol for isolating, culturing, transfecting, and differentiating human primary monocytes from HIV-infected individuals and healthy controls.

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Primary Human MonocytesIsolationTransfectionCultureHIV InfectionImmune DysfunctionAntiretroviral TherapyMolecular MechanismsBiosafetyEDTAMagnetic BeadsPBSCentrifugationCell Counting

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Plaisance-Bonstaff, K., Faia, C., Wyczechowska, D., Jeansonne, D., Vittori, C., Peruzzi, F. Isolation, Transfection, and Culture of Primary Human Monocytes. J. Vis. Exp. (154), e59967, doi:10.3791/59967 (2019).

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