Isolation, Transfection, and Culture of Primary Human Monocytes

Karlie Plaisance-Bonstaff; Celeste Faia; Dorota Wyczechowska; Duane Jeansonne; Cecilia Vittori; Francesca Peruzzi

doi:10.3791/59967

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免疫学と感染

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Isolation, Transfection, and Culture of Primary Human Monocytes

JoVE Journal
免疫学と感染

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JoVE Journal 免疫学と感染

Isolation, Transfection, and Culture of Primary Human Monocytes

12,771 Views

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09:13 min

•

December 16, 2019

DOI:

10.3791/59967-v

DOI:

10.3791/59967-v

•

09:13 min

December 16, 2019

•

17 Views

Karlie Plaisance-Bonstaff^1,2, Celeste Faia², Dorota Wyczechowska¹, Duane Jeansonne¹, Cecilia Vittori^1,3, Francesca Peruzzi^1,2

¹医学部,Louisiana State University Health Sciences Center, ²Department of Microbiology, Immunology, and Parasitology,Louisiana State University Health Sciences Center, ³Department of Biomedical and Clinical Sciences L. Sacco,University of Milan

概要

Presented here is an optimized protocol for isolating, culturing, transfecting, and differentiating human primary monocytes from HIV-infected individuals and healthy controls.

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Isolation, Transfection, and Culture of Primary Human Monocytes

概要

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