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Immunology and Infection

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原発性ヒト単球の単離、トランスフェクション、培養

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation, Transfection, and Culture of Primary Human Monocytes

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原発性ヒト単球の単離、トランスフェクション、培養

12,718 Views

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09:13 min

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December 16, 2019

DOI:

10.3791/59967-v

DOI:

10.3791/59967-v

•

09:13 min

December 16, 2019

•

17 Views

Karlie Plaisance-Bonstaff^1,2, Celeste Faia², Dorota Wyczechowska¹, Duane Jeansonne¹, Cecilia Vittori^1,3, Francesca Peruzzi^1,2

¹Department of Medicine,Louisiana State University Health Sciences Center, ²Department of Microbiology, Immunology, and Parasitology,Louisiana State University Health Sciences Center, ³Department of Biomedical and Clinical Sciences L. Sacco,University of Milan

Summary

ここでは、HIVに感染した個体および健全なコントロールからヒト一次単球を分離、培養、トランスフェクション、および区別するための最適化されたプロトコルです。

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