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Floresan Problar ve Lignin Bitki Kesitlerinde Doğal Otofloresan Dayalı SFLIM Ile Etkileşimlerinin Ölçülmesi
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
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Floresan Problar ve Lignin Bitki Kesitlerinde Doğal Otofloresan Dayalı SFLIM Ile Etkileşimlerinin Ölçülmesi

Türkçe

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00:07 min

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January 02, 2020

DOI:

10.3791/59925-v

DOI:
10.3791/59925-v

•

00:07 min
January 02, 2020

•

2 Views
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1PICT Platform,Université de Reims Champagne-Ardenne, 2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA,Université de Reims Champagne-Ardenne, 3Unité de Glycobiologie Structurale et Fonctionnelle,Université de Lille

Summary

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Bu protokol, förster rezonans enerji transferini (FRET) rhodamine bazlı floresan problar ve lignin polimeri arasında doğrudan kalın bitki bölümlerinde değerlendirmek için spektral ve floresan ömür ölçümlerini birleştiren orijinal bir kurulum tanımlamaktadır.

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Terryn, C., Habrant, A., Paës, G., Spriet, C. Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence. J. Vis. Exp. (155), e59925, doi:10.3791/59925 (2020).

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