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再発作用ポテンシャル記録のためのマルチウェルマイクロ電極アレイ上のヒトiPSC由来心筋細胞ネットワーク
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings
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再発作用ポテンシャル記録のためのマルチウェルマイクロ電極アレイ上のヒトiPSC由来心筋細胞ネットワーク

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11,144 Views

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08:53 min

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July 15, 2019

DOI:

10.3791/59906-v

DOI:
10.3791/59906-v

•

08:53 min
July 15, 2019

•

4 Views
, , , ,
1Aurora Research Institute,Advocate Aurora Health Care, 2Department of Biomedical Engineering, College of Engineering and Applied Science,University of Wisconsin-Milwaukee, 3Department of Medicine-Cardiovascular, School of Medicine,Johns Hopkins University

Summary

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本稿では、マルチウェルMEAプレート上で培養されたヒト誘導多能性幹細胞由来心筋細胞(hiPSC-CM)ネットワークの開発のためのプロトコルのセットを含み、作用電位測定のために細胞膜を可逆的に電気ポレートする。ハイスループットの記録は、同じセルサイトから何日も繰り返し取得されます。

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Cite this Article

Zlochiver, V., Kroboth, S. L., Beal, C. R., Cook, J. A., Joshi-Mukherjee, R. Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings. J. Vis. Exp. (149), e59906, doi:10.3791/59906 (2019).

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