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用于循环作用电位记录的多井微电极阵列上的人类 iPSC 衍生心肌细胞网络
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings
Please note that all translations are automatically generated. Click here for the English version.

用于循环作用电位记录的多井微电极阵列上的人类 iPSC 衍生心肌细胞网络

English

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11,136 Views

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08:53 min

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July 15, 2019

DOI:

10.3791/59906-v

DOI:
10.3791/59906-v

•

08:53 min
July 15, 2019

•

4 Views
, , , ,
1Aurora Research Institute,Advocate Aurora Health Care, 2Department of Biomedical Engineering, College of Engineering and Applied Science,University of Wisconsin-Milwaukee, 3Department of Medicine-Cardiovascular, School of Medicine,Johns Hopkins University

Summary

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本文包含一套用于开发人类诱导多能干细胞衍生心肌细胞(hiPSC-CM)网络的协议,这些网络在多孔MEA板上培养,可逆电化细胞膜进行作用电势测量。高通量记录在几天内从同一单元站点重复获取。

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Cite this Article

Zlochiver, V., Kroboth, S. L., Beal, C. R., Cook, J. A., Joshi-Mukherjee, R. Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings. J. Vis. Exp. (149), e59906, doi:10.3791/59906 (2019).

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