Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

Robert  S. Brzozowski; Maria  L. White; Prahathees J. Eswara

doi:10.3791/59905

/

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

6,734 Views

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05:57 min

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November 23, 2019

DOI:

10.3791/59905-v

DOI:

10.3791/59905-v

•

05:57 min

November 23, 2019

•

7 Views

Robert S. Brzozowski¹, Maria L. White¹, Prahathees J. Eswara¹

¹Department of Cell Biology, Microbiology and Molecular Biology,University of South Florida

Summary

This article provides a step-by-step guide to investigate protein subcellular localization dynamics and to monitor morphological changes using high-resolution fluorescence microscopy in Bacillus subtilis and Staphylococcus aureus.

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Live-cell Fluorescence Microscopy Subcellular Protein Localization Cell Morphology Changes Bacteria Sample Preparation Membrane Staining Dye Agarose Slab High-resolution Deconvolution Microscope 100x Oil Immersion Objective Resolve 3D Imaging Software Z-stacks Sample Thickness

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Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

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