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Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
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Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

6,734 Views

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05:57 min

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November 23, 2019

DOI:

10.3791/59905-v

DOI:
10.3791/59905-v

•

05:57 min
November 23, 2019

•

7 Views
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1Department of Cell Biology, Microbiology and Molecular Biology,University of South Florida

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This article provides a step-by-step guide to investigate protein subcellular localization dynamics and to monitor morphological changes using high-resolution fluorescence microscopy in Bacillus subtilis and Staphylococcus aureus.

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Brzozowski, R. S., White, M. L., Eswara, P. J. Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria. J. Vis. Exp. (153), e59905, doi:10.3791/59905 (2019).

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