This article provides a step-by-step guide to investigate protein subcellular localization dynamics and to monitor morphological changes using high-resolution fluorescence microscopy in Bacillus subtilis and Staphylococcus aureus.
Brzozowski, R. S., White, M. L., Eswara, P. J. Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria. J. Vis. Exp. (153), e59905, doi:10.3791/59905 (2019).