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In vitro generatie van muis Hartveld-specifieke hart voorlopercellen
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells
DOI:

09:29 min

July 03, 2019

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Chapters

  • 00:04Title
  • 00:44Mouse Embryonic Stem Cell (mESC) Maintenance
  • 01:45Cardiac Progenitor Cell (CPC) Generation
  • 03:13Heart Field-specific CPC Isolation by Fluorescent Protein Reporter Expression
  • 04:22Heart Field-specific CPC Isolation by Cell Surface Protein Marker Expression
  • 05:26Heart Field-Specific CPC Analysis
  • 07:18Results: Representative CPC Differentiation Characterization
  • 08:57Conclusion

Summary

Automatic Translation

Het doel van deze methode is het genereren van hartgebied-specifieke cardiale stamcellen in vitro om de stamvader cel specificatie en functionele eigenschappen te bestuderen, en het genereren van kamer specifieke cardiale cellen voor hart-en vaatziekten modellering.

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