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Developmental Biology
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小鼠心脏场特定心脏祖细胞的体外生成
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells
Please note that all translations are automatically generated.
Click here for the English version.
小鼠心脏场特定心脏祖细胞的体外生成
DOI:
10.3791/59826-v
•
09:29 min
•
July 03, 2019
•
Emmanouil Tampakakis
,
Matthew Miyamoto
,
Chulan Kwon
1
Division of Cardiology, Department Medicine
,
Johns Hopkins School of Medicine
Chapters
00:04
Title
00:44
Mouse Embryonic Stem Cell (mESC) Maintenance
01:45
Cardiac Progenitor Cell (CPC) Generation
03:13
Heart Field-specific CPC Isolation by Fluorescent Protein Reporter Expression
04:22
Heart Field-specific CPC Isolation by Cell Surface Protein Marker Expression
05:26
Heart Field-Specific CPC Analysis
07:18
Results: Representative CPC Differentiation Characterization
08:57
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方法的目的是在体外生成心脏场特异性心脏祖细胞,以研究祖细胞的规范和功能特性,并生成用于心脏病建模的腔内特定心脏细胞。
Tags
Heart Field-specific Cardiac Progenitor Cells
Mouse Embryonic Stem Cells
Congenital Heart Disease
Cardiogenesis
Cardiac Spheroids
Activin A
Bone Morphogenetic Protein 4
SFD Medium
2i Medium
In Vitro Generation
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