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小鼠心脏场特定心脏祖细胞的体外生成
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells
DOI:

09:29 min

July 03, 2019

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Chapters

  • 00:04Title
  • 00:44Mouse Embryonic Stem Cell (mESC) Maintenance
  • 01:45Cardiac Progenitor Cell (CPC) Generation
  • 03:13Heart Field-specific CPC Isolation by Fluorescent Protein Reporter Expression
  • 04:22Heart Field-specific CPC Isolation by Cell Surface Protein Marker Expression
  • 05:26Heart Field-Specific CPC Analysis
  • 07:18Results: Representative CPC Differentiation Characterization
  • 08:57Conclusion

Summary

Automatic Translation

该方法的目的是在体外生成心脏场特异性心脏祖细胞,以研究祖细胞的规范和功能特性,并生成用于心脏病建模的腔内特定心脏细胞。

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