Journal
/
Immunology and Infection
/
Eencellige screening methode voor de selectie en terugwinning van antilichamen met de gewenste specifieke kenmerken van verrijkte menselijke geheugen B-celpopulaties
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Please note that all translations are automatically generated. Click here for the English version.

Eencellige screening methode voor de selectie en terugwinning van antilichamen met de gewenste specifieke kenmerken van verrijkte menselijke geheugen B-celpopulaties

Nederlands

Automatically Generated

10,233 Views

•

00:09 min

•

August 22, 2019

DOI:

10.3791/59809-v

DOI:
10.3791/59809-v

•

00:09 min
August 22, 2019

•

16 Views
, , , ,
1World Without Disease Accelerator,Janssen Pharmaceutical Companies of Johnson and Johnson, 2Janssen Prevention Center,Janssen Pharmaceutical Companies of Johnson and Johnson

Summary

Automatically generated

De BSelex-methode om individuele antigeen-specifieke antilichamen van humane perifere bloed mononucleaire cellen te identificeren en te herstellen combineert Flowcytometrie met eencellige PCR en klonen.

Explore More Videos

Single-cell ScreeningAntibody SelectionMemory B CellsHuman Immune RepertoireAntigen-specific B CellsNatively-paired Heavy And Light ChainsCD22-positive B CellsCD19 CD27 AntibodyBiotin Streptavidin TetramersFlow CytometryCell SortingDAPI Staining

Read Article

Cite this Article

Perry, S. T., Keogh, E., Morton, M., Koudstaal, W., Pascual, G. Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations. J. Vis. Exp. (150), e59809, doi:10.3791/59809 (2019).

Download .ris file

Copy

Share Video

.

Copy