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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

DOI:
10.3791/59809-v

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00:09 min

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August 22, 2019

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, , , ,
1World Without Disease Accelerator,Janssen Pharmaceutical Companies of Johnson and Johnson, 2Janssen Prevention Center,Janssen Pharmaceutical Companies of Johnson and Johnson

Bölümler

  • 00:04Başlık
  • 00:30Cell Sorting
  • 03:11Single Cell Cloning
  • 05:41Antigen-Specificity Confirmation by ELISA Screening
  • 06:57Results: Representative Antibody Recovery and Antigen-Specificity Confirmation
  • 08:30Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The BSelex method to identify and recover individual antigen-specific antibodies from human peripheral blood mononuclear cells combines flow cytometry with single cell PCR and cloning.

Etiketler

Single-cell ScreeningAntibody SelectionMemory B CellsHuman Immune RepertoireAntigen-specific B CellsNatively-paired Heavy And Light ChainsCD22-positive B CellsCD19 CD27 AntibodyBiotin Streptavidin TetramersFlow CytometryCell SortingDAPI Staining

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