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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

10,258 Views

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00:09 min

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August 22, 2019

DOI:

10.3791/59809-v

DOI:
10.3791/59809-v

•

00:09 min
August 22, 2019

•

16 Views
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1World Without Disease Accelerator,Janssen Pharmaceutical Companies of Johnson and Johnson, 2Janssen Prevention Center,Janssen Pharmaceutical Companies of Johnson and Johnson

Summary

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The BSelex method to identify and recover individual antigen-specific antibodies from human peripheral blood mononuclear cells combines flow cytometry with single cell PCR and cloning.

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Perry, S. T., Keogh, E., Morton, M., Koudstaal, W., Pascual, G. Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations. J. Vis. Exp. (150), e59809, doi:10.3791/59809 (2019).

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