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Sintesi ezimatica dei metaboliti epossidici degli acidi docosahexaenoici, eicosapentaenoici e aracidonici
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Chemistry
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JoVE Journal Chemistry
Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids
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Sintesi ezimatica dei metaboliti epossidici degli acidi docosahexaenoici, eicosapentaenoici e aracidonici

italiano

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7,722 Views

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13:05 min

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June 28, 2019

DOI:

10.3791/59770-v

DOI:
10.3791/59770-v

•

13:05 min
June 28, 2019

•

12 Views
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1Department of Pharmacology and Toxicology,Michigan State University

Summary

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Presentiamo un metodo utile per la sintesi e purificazione e depurazione esulla scala di specifici enantiomeri e regioisori di epossido di acido aracidonico (AA), acido docosaesaenoico (DHA) e acido eicosapentaeno (EPA) con l'uso di un citocromo batterico P450 enzima (BM3).

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Enzymatic SynthesisEpoxidized MetabolitesDocosahexaenoic Acid (DHA)Eicosapentaenoic Acid (EPA)Arachidonic AcidEnantiopure Epoxy Fatty AcidsEnzyme PurificationCell LysisAffinity Chromatography

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Cite this Article

Woodman, J. W., Cinelli, M. A., Scharmen-Burgdolf, A., Lee, K. S. S. Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids. J. Vis. Exp. (148), e59770, doi:10.3791/59770 (2019).

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