È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids
7,722 Views
•
13:05 min
•
June 28, 2019
DOI:
Summary
Automatically generated
We present a method useful for large-scale enzymatic synthesis and purification of specific enantiomers and regioisomers of epoxides of arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) with the use of a bacterial cytochrome P450 enzyme (BM3).
Read Article
Cite this Article
Woodman, J. W., Cinelli, M. A., Scharmen-Burgdolf, A., Lee, K. S. S. Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids. J. Vis. Exp. (148), e59770, doi:10.3791/59770 (2019).
Copy