Dergi
/
Kimya
/
Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids
JoVE Journal
Kimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Kimya
Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids

Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids

DOI:
10.3791/59770-v

•

13:05 min

•

June 28, 2019

•

, , ,
1Department of Pharmacology and Toxicology,Michigan State University

Bölümler

  • 00:04Başlık
  • 00:52Expression of Wild-type BM3 (Bacterial Cytochrome P450 Enzyme)
  • 02:10Purification of BM3
  • 05:35Extraction of EDPs (Epoxydocosapentaenoic Acids)
  • 07:23Esterification of EDPs, Separation of 16(S), 17(R)- and 19(S),20(R)-EDP, and Saponification of Esters
  • 11:17Results: Chromatogram and 1H-NMR Spectra
  • 12:13Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We present a method useful for large-scale enzymatic synthesis and purification of specific enantiomers and regioisomers of epoxides of arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) with the use of a bacterial cytochrome P450 enzyme (BM3).

Etiketler

Enzymatic SynthesisEpoxidized MetabolitesDocosahexaenoic Acid (DHA)Eicosapentaenoic Acid (EPA)Arachidonic AcidEnantiopure Epoxy Fatty AcidsEnzyme PurificationCell LysisAffinity Chromatography

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Synthesis and Characterization of Functionalized Metal-organic Frameworks

Synthesis and Reaction Chemistry of Nanosize Monosodium Titanate

Light-driven Enzymatic Decarboxylation

Synthesis and Characterization of Supramolecular Colloids

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine

Synthesis of Substrate-Bound Au Nanowires Via an Active Surface Growth Mechanism

Synthesis and Characterization of Amphiphilic Gold Nanoparticles

Read Article