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एक पर झिल्ली पाचन तकनीक का उपयोग कर प्रोटीन-प्रोटीन इंटरेक्शन का मूल्यांकन

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

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एक पर झिल्ली पाचन तकनीक का उपयोग कर प्रोटीन-प्रोटीन इंटरेक्शन का मूल्यांकन

6,367 Views

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07:07 min

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July 19, 2019

DOI:

10.3791/59733-v

DOI:

10.3791/59733-v

•

07:07 min

July 19, 2019

•

6 Views

Takashi Obama*¹, Takuro Miyazaki*², Toshihiro Aiuchi, Akira Miyazaki, Hiroyuki Itabe

¹Division of Biological Chemistry, Department of Molecular Biology,Showa University School of Pharmacy, ²Department of Biochemistry,Showa University School of Medicine

Tags

Protein-protein Interaction On-membrane Digestion Proteomic Analysis Immunoprecipitation Magnetic Beads PVDF Membrane Mass Spectrometry

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