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Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
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JoVE Journal Biyokimya
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

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07:07 min

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July 19, 2019

DOI:

10.3791/59733-v

DOI:
10.3791/59733-v

•

07:07 min
July 19, 2019

•

1 Views
, , , ,
1Division of Biological Chemistry, Department of Molecular Biology,Showa University School of Pharmacy, 2Department of Biochemistry,Showa University School of Medicine

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Here, we present a protocol for an on-membrane digestion technique for the preparation of samples for mass spectrometry. This technique facilitates the convenient analysis of protein–protein interactions.

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Obama, T., Miyazaki, T., Aiuchi, T., Miyazaki, A., Itabe, H. Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique. J. Vis. Exp. (149), e59733, doi:10.3791/59733 (2019).

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