Here, we present a protocol for an on-membrane digestion technique for the preparation of samples for mass spectrometry. This technique facilitates the convenient analysis of protein–protein interactions.
Obama, T., Miyazaki, T., Aiuchi, T., Miyazaki, A., Itabe, H. Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique. J. Vis. Exp. (149), e59733, doi:10.3791/59733 (2019).