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用于大分子 X 射线晶体学的一体式样品支架,具有最小的背景散射
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
DOI:

07:55 min

July 06, 2019

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Chapters

  • 00:04Title
  • 00:37Setting up Crystallization Drops and Observing Crystal Growth
  • 01:56Crystal Manipulation
  • 04:23In Situ Diffraction Data Collection at Ambient Temperature and at Cryogenic Temperature
  • 06:20Results: Novel Sample Holder in Macromolecular X-Ray Crystallography for Crystal Growth, Crystal Manipulation, and X-Ray Diffraction Data Collection
  • 07:23Conclusion

Summary

Automatic Translation

提出了一种用于大分子X射线晶体学的新型样品架以及合适的处理方案。该系统允许在环境温度和低温温度下采集晶体生长、晶体浸泡和原位衍射数据,无需任何晶体操作或安装。

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