A novel sample holder for macromolecular X-ray crystallography along with a suitable handling protocol is presented. The system allows crystal growth, crystal soaking and in situ diffraction data collection at both, ambient and cryogenic temperature without the need of any crystal manipulation or mounting.
Feiler, C. G., Wallacher, D., Weiss, M. S. An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering. J. Vis. Exp. (149), e59722, doi:10.3791/59722 (2019).