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An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
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Biochemie
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JoVE Journal Biochemie
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

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07:55 min

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July 06, 2019

DOI:

10.3791/59722-v

DOI:
10.3791/59722-v

•

07:55 min
July 06, 2019

•

10 Views
, ,
1Macromolecular Crystallography (HZB-MX),Helmholtz-Zentrum Berlin, 2Department Sample Environment,Helmholtz-Zentrum Berlin

Summary

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A novel sample holder for macromolecular X-ray crystallography along with a suitable handling protocol is presented. The system allows crystal growth, crystal soaking and in situ diffraction data collection at both, ambient and cryogenic temperature without the need of any crystal manipulation or mounting.

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Feiler, C. G., Wallacher, D., Weiss, M. S. An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering. J. Vis. Exp. (149), e59722, doi:10.3791/59722 (2019).

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