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Bir bakteriyel yapay kromozom cDNA klon rekombinant Zika virüs kurtarma
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
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Bir bakteriyel yapay kromozom cDNA klon rekombinant Zika virüs kurtarma

Türkçe

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26,358 Views

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08:10 min

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June 24, 2019

DOI:

10.3791/59537-v

DOI:
10.3791/59537-v

•

08:10 min
June 24, 2019

•

5 Views
, , , ,
1Department of Microbiology and Immunology,University of Rochester Medical Center, 2Department of Molecular and Cell Biology,Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

Summary

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Zika virüsü son salgını aşılar ve/veya terapötik stratejileri geliştirmek için ters genetik yaklaşımlar kurulması önemini vurgular. Burada, biz insan sitomegalovirus acil-erken Organizatör kontrolü altında bir bakteriyel yapay kromozom monte tam uzunlukta cDNA klon bir bulaşıcı rekombinant Zika virüsü kurtarmak için protokol tarif.

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Cite this Article

Ávila-Pérez, G., Park, J., Nogales, A., Almazán, F., Martínez-Sobrido, L. Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone. J. Vis. Exp. (148), e59537, doi:10.3791/59537 (2019).

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