Journal
/
Immunology and Infection
/
Resgate do vírus Zika recombinante de um clone de cDNA de cromossomo artificial bacteriano
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone

Resgate do vírus Zika recombinante de um clone de cDNA de cromossomo artificial bacteriano

português

Automatically Generated

26,358 Views

•

08:10 min

•

June 24, 2019

DOI:

10.3791/59537-v

DOI:
10.3791/59537-v

•

08:10 min
June 24, 2019

•

5 Views
, , , ,
1Department of Microbiology and Immunology,University of Rochester Medical Center, 2Department of Molecular and Cell Biology,Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

Summary

Automatically generated

A recente epidemia do vírus Zika destaca a importância de estabelecer abordagens genéticas inversas para desenvolver vacinas e/ou estratégias terapêuticas. Aqui, nós descrevemos o protocolo para resgatar um vírus de recombinação contagioso do Zika de um clone full-length do cDNA montado em um cromossoma artificial bacteriano o controle do promotor imediato-adiantado humano do citomegalovírus.

Explore More Videos

Zika VirusReverse GeneticsBacterial Artificial ChromosomeCDNA CloneInfectious CloneRNA VirusFlavivirusViral BiologyViral PathogenesisVaccine DevelopmentTransfectionVero Cells

Read Article

Cite this Article

Ávila-Pérez, G., Park, J., Nogales, A., Almazán, F., Martínez-Sobrido, L. Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone. J. Vis. Exp. (148), e59537, doi:10.3791/59537 (2019).

Download .ris file

Copy

Share Video

.

Copy