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Immunologie und Infektion
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Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone

Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone

26,358 Views

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08:10 min

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June 24, 2019

DOI:

10.3791/59537-v

DOI:
10.3791/59537-v

•

08:10 min
June 24, 2019

•

5 Views
, , , ,
1Department of Microbiology and Immunology,University of Rochester Medical Center, 2Department of Molecular and Cell Biology,Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

Summary

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The recent epidemic of Zika virus highlights the importance of establishing reverse genetic approaches to develop vaccines and/or therapeutic strategies. Here, we describe the protocol to rescue an infectious recombinant Zika virus from a full-length cDNA clone assembled in a bacterial artificial chromosome under the control of the human cytomegalovirus immediate-early promoter.

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Zika VirusReverse GeneticsBacterial Artificial ChromosomeCDNA CloneInfectious CloneRNA VirusFlavivirusViral BiologyViral PathogenesisVaccine DevelopmentTransfectionVero Cells

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Cite this Article

Ávila-Pérez, G., Park, J., Nogales, A., Almazán, F., Martínez-Sobrido, L. Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone. J. Vis. Exp. (148), e59537, doi:10.3791/59537 (2019).

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