Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Melvin Noe Gonzalez; Joan  W. Conaway; Ronald  C. Conaway

doi:10.3791/59497

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Complexos artificiais de alongamento de RNA polimerase II para dissecar eventos co-transcricionais do processamento de RNA

JoVE Journal
Genetics

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JoVE Journal Genetics

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Complexos artificiais de alongamento de RNA polimerase II para dissecar eventos co-transcricionais do processamento de RNA

9,558 Views

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10:59 min

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May 13, 2019

DOI:

10.3791/59497-v

DOI:

10.3791/59497-v

•

10:59 min

May 13, 2019

•

2 Views

Melvin Noe Gonzalez¹, Joan W. Conaway^1,2, Ronald C. Conaway^1,2

¹Stowers Institute for Medical Research, ²Department of Biochemistry and Molecular Biology,Kansas University Medical Center

Summary

Aqui, nós descrevemos o conjunto de complexos do alongamento do polymerase II (pol II) do RNA que exigem somente o ADN sintético curto e oligonucleotídeos do RNA e o pol purificado II. Esses complexos são úteis para estudar mecanismos subjacentes ao processamento cotranscricional de transcrições associadas ao complexo de alongamento pol II.

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Complexos artificiais de alongamento de RNA polimerase II para dissecar eventos co-transcricionais do processamento de RNA

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