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Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
Chapters
- 00:04Title
- 00:31Assembly of Artificial Elongation Complexes
- 04:36Walking Pol II to make 23, 25, and 29 Nucleotide Transcripts
- 06:30Using Artificial Elongation Complexes to Assay Co-transcriptional Capping
- 09:01Results: Artificial Elongation Complexes and Co-transcriptional CTD Phosphorylation-dependent RNA Capping
- 10:26Conclusion
Here, we describe the assembly of RNA polymerase II (Pol II) elongation complexes requiring only short synthetic DNA and RNA oligonucleotides and purified Pol II. These complexes are useful for studying mechanisms underlying co-transcriptional processing of transcripts associated with the Pol II elongation complex.
