Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
9,558 Views
•
10:59 min
•
May 13, 2019
DOI:
Summary
Automatically generated
Here, we describe the assembly of RNA polymerase II (Pol II) elongation complexes requiring only short synthetic DNA and RNA oligonucleotides and purified Pol II. These complexes are useful for studying mechanisms underlying co-transcriptional processing of transcripts associated with the Pol II elongation complex.
Read Article
Cite this Article
Noe Gonzalez, M., Conaway, J. W., Conaway, R. C. Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events. J. Vis. Exp. (147), e59497, doi:10.3791/59497 (2019).
Copy