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Multi-locus Variable-number Tandem-repeat Analyse des Fischpathogenen Bakteriums Yersinia ruckeri durch Multiplex-PCR und Kapillarelektrophorese
JoVE Journal
Genetics
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JoVE Journal Genetics
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
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Multi-locus Variable-number Tandem-repeat Analyse des Fischpathogenen Bakteriums Yersinia ruckeri durch Multiplex-PCR und Kapillarelektrophorese

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10,462 Views

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10:33 min

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June 17, 2019

DOI:

10.3791/59455-v

DOI:
10.3791/59455-v

•

10:33 min
June 17, 2019

•

3 Views
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1Fish Health Research Group,Norwegian Veterinary Institute, 2University of Bergen

Summary

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Der hier vorgestellte Multi-Locus Variable-number Tandem-Repeat Analysis (MLVA)-Assay ermöglicht eine kostengünstige, robuste und tragbare hochauflösende Genotypisierung des fischpathogenen Bakteriums Yersinia ruckeri. Ausgehend von reinen Kulturen verwendet der Assay Multiplex-PCR- und Kapillarelektrophorese, um zehn Loci MLVA-Profile für nachgelagerte Anwendungen zu erstellen.

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Gulla, S., Mohammad, S. N., Colquhoun, D. J. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (148), e59455, doi:10.3791/59455 (2019).

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