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Multi-Locus variabele-nummer tandem-herhaling analyse van de vis-pathogene bacterie Yersinia ruckeri door multiplex PCR en capillaire elektroforese
JoVE Journal
Genetics
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JoVE Journal Genetics
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
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Multi-Locus variabele-nummer tandem-herhaling analyse van de vis-pathogene bacterie Yersinia ruckeri door multiplex PCR en capillaire elektroforese

Nederlands

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10,406 Views

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10:33 min

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June 17, 2019

DOI:

10.3791/59455-v

DOI:
10.3791/59455-v

•

10:33 min
June 17, 2019

•

3 Views
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1Fish Health Research Group,Norwegian Veterinary Institute, 2University of Bergen

Summary

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De multi-Locus variabele-aantal tandem-repeat analyse (MLVA) assay hier gepresenteerd maakt goedkope, robuuste en draagbare hoge-resolutie genotypering van de vis-pathogene bacterie Yersinia ruckeri. Beginnend bij zuivere culturen, gebruikt de assay multiplex PCR en capillaire elektroforese om tien-loci MLVA-profielen voor downstreamtoepassingen te produceren.

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Cite this Article

Gulla, S., Mohammad, S. N., Colquhoun, D. J. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (148), e59455, doi:10.3791/59455 (2019).

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