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I Vitro Generation af Somite derivater fra menneskelige induceret celler pluripotente stamceller
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

I Vitro Generation af Somite derivater fra menneskelige induceret celler pluripotente stamceller

DOI:
10.3791/59359-v

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06:21 min

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April 25, 2019

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, ,
1Department of Life Science Frontiers, Center for iPS Cells Research and Application,Kyoto University, 2Department of Clinical Application, Center for iPS Cells Research and Application,Kyoto University

Chapters

  • 00:04Title
  • 00:58Human Induced Pluripotent Stem Cell (iPSC) Pre-induction Preparation
  • 02:14Presomitic Mesoderm (PSM) and Somitic (SM) Differentiation
  • 03:20SM Derivative [Syndetome (SYN)] Differentiation
  • 04:28Results: Representative iPSC-derived Product Characterization
  • 05:44Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer her en protokol for differentiering af menneskelige inducerede pluripotente stamceller i hver somite derivat (myotome, sclerotome, dermatome og syndetome) i kemisk definerede betingelser, som har applikationer i fremtidige sygdom modellering og celle-baserede behandlinger i ortopædisk kirurgi.

Tags

Induced Pluripotent Stem CellsIPSCSomite DerivativesMyotomeDermatomeSclerotomeSyndetomeParaxial MesodermPresomitic MesodermDelta-like Protein 1Regenerative TherapyDisease ModelingMusculoskeletal DisorderHuman Embryogenesis

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