In Vivo Tri-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette In Vivo Tri-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette In Vivo
In Vivo Tri-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette In Vivo Tri-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette In Vivo
Nous présentons une procédure d'éjection locale optimisée utilisant une micropipette en verre et une méthode rapide d'imagerie d'hyperpile de deux photons, qui permet la mesure précise des changements de diamètre capillaire et l'étude de sa régulation en trois dimensions.
Cai, C., Zambach, S. A., Fordsmann, J. C., Lønstrup, M., Thomsen, K. J., Jensen, A. G. K., Lauritzen, M. In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette. J. Vis. Exp. (148), e59286, doi:10.3791/59286 (2019).