Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / In Vivo Three-Dimensional Two-Photon Microscopy…
JoVE Journal
Neuroscience

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Neuroscience
In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette
 

In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

Article DOI: 10.3791/59286-v • 10:54 min • June 7th, 2019
June 7th, 2019 •
1, 1, 1, 1, 1, 1, 1,2
1Department of Neuroscience, Faculty of Medicine and Health Science, University of Copenhagen, 2Department of Clinical Neurophysiology, Rigshospitalet

Kapitel

Summary
Automatic Translation

We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.

Tags

In Vivo Three-Dimensional Two-Photon Microscopy Conducted Vascular Responses Local ATP Ejection Glass Micro-Pipette Slow Conducted Vascular Responses Cerebral Capillaries Local Synaptic Activities 3D Two-photon Microscopy Cerebral Neuroma Glial Activity Brain Function Oxygen And Nutrition Supply Complex Network Of Vessels Cerebral Blood Flow (CBF) Capillary Level In Vivo Two-photon Microscopy Studies CBF Responses Localized Interventions Vascular Network Hyperstack Two-photon Imaging
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter