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In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette
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JoVE Journal 神経科学
In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

7,337 Views

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10:54 min

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June 07, 2019

DOI:

10.3791/59286-v

DOI:
10.3791/59286-v

•

10:54 min
June 07, 2019

•

3 Views
, , , , , ,
1Department of Neuroscience, Faculty of Medicine and Health Science,University of Copenhagen, 2Department of Clinical Neurophysiology,Rigshospitalet

概要

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We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.

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Cite this Article

Cai, C., Zambach, S. A., Fordsmann, J. C., Lønstrup, M., Thomsen, K. J., Jensen, A. G. K., Lauritzen, M. In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette. J. Vis. Exp. (148), e59286, doi:10.3791/59286 (2019).

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