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Genetics
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remtoe 控制系统在内源基因表达调控中的应用
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Please note that all translations are automatically generated.
Click here for the English version.
remtoe 控制系统在内源基因表达调控中的应用
DOI:
10.3791/59235-v
•
08:54 min
•
March 29, 2019
•
Nicole A. Vander Schaaf
,
Shirley Oghamian
,
Jin-A Park
,
Liang Kang
,
Peter W. Laird
,
Kwang-Ho Lee
1
Center for Epigenetics
,
Van Andel Research Institute
,
2
Amgen
Chapters
00:04
Title
00:40
Overview of the REMOTE-control System
01:50
Modify the Gene of Interest for Repression by REMOTE-control
06:04
Manipulate Gene Expression In Vivo
06:50
Results: In vivo Repression and Activation Capability of the REMOTE-control System
08:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议概述了生成模型系统所需的步骤, 在该模型系统中, 相关内源基因的转录可以在活体动物或细胞中使用增强
的 lac 抑制和/
或 tet
激活剂系统有条件地控制。
Tags
REMOTE-control System
Endogenous Gene Expression
Gene Function
Gene Repression
Gene Upregulation
LacIGY Repressor
RtTA-M2 Activator
IPTG
Doxycycline
Gene Modification
UCSC Genome Browser
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