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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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Genetik
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JoVE Journal Genetik
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

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08:54 min

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March 29, 2019

DOI:

10.3791/59235-v

DOI:
10.3791/59235-v

•

08:54 min
March 29, 2019

•

1 Views
, , , , ,
1Center for Epigenetics,Van Andel Research Institute, 2Amgen

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This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator systems.

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Vander Schaaf, N. A., Oghamian, S., Park, J., Kang, L., Laird, P. W., Lee, K. In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression. J. Vis. Exp. (145), e59235, doi:10.3791/59235 (2019).

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