Dergi
/
Genetik
/
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
JoVE Journal
Genetik
This content is Free Access.
JoVE Journal Genetik
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

6,685 Views

•

08:54 min

•

March 29, 2019

DOI:

10.3791/59235-v

DOI:
10.3791/59235-v

•

08:54 min
March 29, 2019

•

1 Views
, , , , ,
1Center for Epigenetics,Van Andel Research Institute, 2Amgen

Özet

Automatically generated

This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator systems.

Explore More Videos

REMOTE-control SystemEndogenous Gene ExpressionGene FunctionGene RepressionGene UpregulationLacIGY RepressorRtTA-M2 ActivatorIPTGDoxycyclineGene ModificationUCSC Genome Browser

Read Article

Cite this Article

Vander Schaaf, N. A., Oghamian, S., Park, J., Kang, L., Laird, P. W., Lee, K. In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression. J. Vis. Exp. (145), e59235, doi:10.3791/59235 (2019).

Download .ris file

Copy

Share Video

.

Copy