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一种在裂变酵母中的深度固序辅助自发抑制屏
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
DOI:

07:55 min

March 07, 2019

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Chapters

  • 00:04Title
  • 00:32Plate Reader Assay
  • 03:13Selection of Suppressor Colonies and Confirmation of Phenotype
  • 05:27Results: Slow-growing Mutants Show Phenotypic Recovery and Genetic Confirmation of Identified Suppressor Mutations Verifies the Heritability of the Recovery Phenotype
  • 06:56Conclusion

Summary

Automatic Translation

我们提出了一个简单的抑制屏幕协议在裂变酵母。这种方法是有效的, 无突变, 并选择性的突变, 经常发生在一个单一的基因组位点。 该协议适用于分离抑制剂, 以减轻由突变或药物引起的液体培养中的生长缺陷。

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