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A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
JoVE Journal
Genética
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JoVE Journal Genética
A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

7,750 Views

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07:55 min

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March 07, 2019

DOI:

10.3791/59133-v

DOI:
10.3791/59133-v

•

07:55 min
March 07, 2019

•

10 Views
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1Department of Biology,Wake Forest University, 2Center for Molecular Communication and Signaling,Wake Forest University

Summary

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We present a simple suppressor screen protocol in fission yeast. This method is efficient, mutagen-free, and  selective  for  mutations that  often  occur at   a  single  genomic  locus.  The protocol is suitable for isolating suppressors that alleviate growth defects in liquid culture that are caused by a mutation or a drug.

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Fission YeastSchizosaccharomyces PombeSuppressor ScreenDeep SequencingSpontaneous MutationGrowth DefectOptical DensityPlate ReaderAutomated DilutionKinetic Growth Curve

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Cite this Article

Marayati, B. F., Pease, J. B., Zhang, K. A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe. J. Vis. Exp. (145), e59133, doi:10.3791/59133 (2019).

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