Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

Seth A. Bennett; Samantha N. Cobos; Marcella Meykler; Michel Fallah; Navin Rana; Karen Chen; Mariana P. Torrente

doi:10.3791/59104

Journal

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Genetics

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酵母神経変性認知症予備モデルにおけるヒストン翻訳後修飾変化の特性

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Genetics

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JoVE Journal Genetics

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

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酵母神経変性認知症予備モデルにおけるヒストン翻訳後修飾変化の特性

7,330 Views

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08:33 min

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March 24, 2019

DOI:

10.3791/59104-v

DOI:

10.3791/59104-v

•

08:33 min

March 24, 2019

•

9 Views

Seth A. Bennett^1,2, Samantha N. Cobos^1,3, Marcella Meykler¹, Michel Fallah¹, Navin Rana¹, Karen Chen¹, Mariana P. Torrente^1,4

¹Chemistry Department,Brooklyn College, ²Ph.D. Program in Biochemistry,Graduate Center of the City University of New York, ³Ph.D. Program in Chemistry,Graduate Center of the City University of New York, ⁴Ph.D. Programs in Chemistry, Biochemistry, and Biology,Graduate Center of the City University of New York

Summary

このプロトコルヒストン翻訳後修飾 (PTM) ALS およびパーキンソン病に関連するタンパク質の過剰発現に関連して発生するレベルのゲノム変化を特徴づける実験手順の概要を説明します。出芽酵母のモデル。SDS ページの分離後個々のヒストン翻訳後修飾レベルは西部にしみが付くことによって修飾特異抗体で検出されます。

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酵母神経変性認知症予備モデルにおけるヒストン翻訳後修飾変化の特性

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Summary

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