Journal
/
Genetics
/
Karakterisering van Histone posttranslationele wijziging wijzigingen in gist neurodegeneratieve Proteinopathy modellen
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
Please note that all translations are automatically generated. Click here for the English version.

Karakterisering van Histone posttranslationele wijziging wijzigingen in gist neurodegeneratieve Proteinopathy modellen

Nederlands

Automatically Generated

7,329 Views

•

08:33 min

•

March 24, 2019

DOI:

10.3791/59104-v

DOI:
10.3791/59104-v

•

08:33 min
March 24, 2019

•

9 Views
, , , , , ,
1Chemistry Department,Brooklyn College, 2Ph.D. Program in Biochemistry,Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry,Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology,Graduate Center of the City University of New York

Summary

Automatically generated

Dit protocol beschrijft experimentele procedures karakteriseren van genoom-brede veranderingen in de niveaus van Histon posttranslationele modificaties (PTM) die zich voordoen in verband met de overexpressie van eiwitten ALS en Parkinson in zijn gekoppeld Saccharomyces cerevisiae modellen. Na SDS-pagina de scheiding, worden individuele Histon PTM niveaus gedetecteerd met wijziging-specifieke antilichamen via het westelijke bevlekken.

Explore More Videos

HistonePost-translational ModificationYeastNeurodegenerative ProteinopathyEpigeneticsNeurodegenerative DiseaseFibroblastsInduced Pluripotent Stem CellsWestern BlotYeast CultureProtein OverexpressionOptical DensityCell HarvestingCell Lysis

Read Article

Cite this Article

Bennett, S. A., Cobos, S. N., Meykler, M., Fallah, M., Rana, N., Chen, K., Torrente, M. P. Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models. J. Vis. Exp. (145), e59104, doi:10.3791/59104 (2019).

Download .ris file

Copy

Share Video

.

Copy