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Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
1Chemistry Department,Brooklyn College, 2Ph.D. Program in Biochemistry,Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry,Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology,Graduate Center of the City University of New York
Capitoli
- 00:04Titolo
- 01:00Neurodegenerative Proteinopathy-associated Protein Overexpression in S. cerevisiae
- 03:02Cell Lysis and Protein Transfer
- 05:28Histone Post-translational Modification Detection
- 06:52Results: Representative Effects of Yeast Protein Overexpression on Histone Expression and Modification
- 08:03Conclusion
This protocol outlines experimental procedures to characterize genome-wide changes in the levels of histone post-translational modifications (PTM) occurring in connection with the overexpression of proteins associated with ALS and Parkinson's disease in Saccharomyces cerevisiae models. After SDS-PAGE separation, individual histone PTM levels are detected with modification-specific antibodies via Western blotting.
