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Immunology and Infection

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用原生动物给鱼作动物感染的载体

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae

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用原生动物给鱼作动物感染的载体

DOI:

10.3791/58949-v

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07:49 min

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January 07, 2019

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Erika Flores, Laurel Thompson, Natalie Sirisaengtaksin, Anh Trinh Nguyen, Abigail Ballard, Anne-Marie Krachler

¹McGovern Medical School, Department of Microbiology and Molecular Genetics,University of Texas Health Science Center at Houston

Chapters

00:04Title
00:40Paramecia Growth and Maintenance and Bacterial Dose Determination
02:23Paramecia Preparation for Food-borne Infection
04:40Zebrafish Food-borne Infection and Pre-rate Determination
06:30Results: Representative Zebrafish Bacterial Colonization Post Paramecia Consumption
07:28Results

Summary

Automatic Translation

斑马鱼 (dadio rerio)正在成为一种广泛使用的脊椎动物动物模型, 用于微生物定植和发病机制。该协议描述了原生动物帕拉米库姆作为一种载体, 在斑马鱼幼虫的食物传播感染的使用。尾状物容易将细菌内化, 并通过自然捕食行为被幼虫斑马鱼占据。

Tags

Paramecium Caudatum Food-borne Infections Zebrafish Larvae Microbiology Gastrointestinal Tract Oral Gavage E. Coli MG1655 Colony Forming Units Non-ionic Surfactant PBS Selective Plates Bacterial Colonies Optical Density Bacterial Stain Photo Bleaching

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