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February 18, 2011
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Methods for bioluminescence imaging of bacterial infections in living animals are decribed. Pathogens are modified to express luciferase allowing optical whole body imaging of infections in live animals. Animal models can be infected with luciferase expressing pathogens and the resulting course of disease visualized in real-time by bioluminescence imaging.
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LuciferaseImagingBacterial InfectionsMiceBioluminescence ImagingReal-time MonitoringCancer CellsStem CellsImmune Cell TypesViral PathogensParasitesConventional StrategiesAnimal ModelsOptical ImagingInoculationVariability Between AnimalsReduce Animal NumbersData CollectionRecombinant Strains Of Bacteria Expressing LuciferaseClick Beetle LuciferaseFirefly Luciferase
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Chang, M. H., Cirillo, S. L., Cirillo, J. D. Using Luciferase to Image Bacterial Infections in Mice. J. Vis. Exp. (48), e2547, doi:10.3791/2547 (2011).
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