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Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices
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Neuroscience
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JoVE Journal Neuroscience
Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices

Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices

English

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9,501 Views

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07:27 min

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January 31, 2019

DOI:

10.3791/58788-v

DOI:
10.3791/58788-v

•

07:27 min
January 31, 2019

•

2 Views
, , , , ,
1Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, 2Sorbonne Université, Paris, France, 3Institut du Fer à Moulin, Paris, France

Summary

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Microglia, the resident immune cells of the brain, respond quickly with morphological changes to modifications of their environment. This protocol describes how to use two-photon microscopy to study the attraction of microglial processes toward serotonin or ATP in acute brain slices of mice.

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Cite this Article

Etienne, F., Mastrolia, V., Maroteaux, L., Girault, J., Gervasi, N., Roumier, A. Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices. J. Vis. Exp. (143), e58788, doi:10.3791/58788 (2019).

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