Journal
/
神经科学
/
Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices

Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices

DOI:
10.3791/58788-v

•

07:27 min

•

January 31, 2019

•

, , , , ,
1Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, 2Sorbonne Université, Paris, France, 3Institut du Fer à Moulin, Paris, France

Chapters

  • 00:04Title
  • 00:42Dissection Area Preparation
  • 01:51Brain Slicing
  • 03:112-photon Live Imaging
  • 04:12Compound Application
  • 06:14Results: Microglial Response Analyses
  • 07:05Conclusion

Summary

自动翻译

自动翻译

Microglia, the resident immune cells of the brain, respond quickly with morphological changes to modifications of their environment. This protocol describes how to use two-photon microscopy to study the attraction of microglial processes toward serotonin or ATP in acute brain slices of mice.

Tags

Keywords: Two-photon ImagingMicroglial ProcessesATPSerotoninAcute Brain SlicesDirectional Motility3D Print InterfacePerfusion ChambersCholine ACSFVibrating SlicerInterface ChamberPeristaltic PumpRecording Chamber

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/58788?article_type=v

Preparation of Acute Subventricular Zone Slices for Calcium Imaging
check_url/cn/58788?article_type=v

In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons
check_url/cn/58788?article_type=v

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices
check_url/cn/58788?article_type=v

Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord
check_url/cn/58788?article_type=v

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
check_url/cn/58788?article_type=v

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
check_url/cn/58788?article_type=v

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
check_url/cn/58788?article_type=v

In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice
check_url/cn/58788?article_type=v

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK
check_url/cn/58788?article_type=v

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

Read Article