Rivista
/
Neuroscienze
/
Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices

Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices

9,501 Views

•

07:27 min

•

January 31, 2019

DOI:

10.3791/58788-v

DOI:
10.3791/58788-v

•

07:27 min
January 31, 2019

•

2 Views
, , , , ,
1Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, 2Sorbonne Université, Paris, France, 3Institut du Fer à Moulin, Paris, France

Summary

Automatically generated

Microglia, the resident immune cells of the brain, respond quickly with morphological changes to modifications of their environment. This protocol describes how to use two-photon microscopy to study the attraction of microglial processes toward serotonin or ATP in acute brain slices of mice.

Explore More Videos

Two-photon ImagingMicroglial ProcessesATPSerotoninAcute Brain SlicesDirectional Motility3D Print InterfacePerfusion ChambersCholine ACSFVibrating SlicerInterface ChamberPeristaltic PumpRecording Chamber

Read Article

Cite this Article

Etienne, F., Mastrolia, V., Maroteaux, L., Girault, J., Gervasi, N., Roumier, A. Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices. J. Vis. Exp. (143), e58788, doi:10.3791/58788 (2019).

Download .ris file

Copy

Share Video

.

Copy