Journal
/
Immunology and Infection
/
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

DOI:
10.3791/58929-v

•

10:26 min

•

January 20, 2019

•

, , , ,
1IGH, CNRS,Univ Montpellier, 2Laboratory for Monitoring Innovative Therapies, Department of Biological Hematology,CHU Montpellier, 3UFR de Médecine,Univ Montpellier

Chapters

  • 00:04Title
  • 00:52B Cell Amplification and Differentiation
  • 03:44Plasmablastic Cell Generation
  • 04:36Early Plasma Cell Generation
  • 05:21Long-lived Plasma Cell Generation
  • 06:29Molecular Atlas of B to PC Differentiation
  • 07:33Results: Epigenetic Factor Expression in Normal Plasma Cell Differentiation
  • 09:01Conclusion

Summary

Automatic Translation

Automatic Translation

Using multi-step culture systems, we report an in vitro B cell to plasma cell differentiation model.

Tags

In Vitro DifferentiationHuman Normal Memory B CellsLong-lived Plasma CellsPlasma Cell DifferentiationB2 Plasma Cell DifferentiationTranscriptional ChangesPhenotypeBioinformatic ToolsMemory B Cell PurificationCD40 LigandAnti-polyhistidine Monoclonal Antibody

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Murine Model of CD40-activation of B cells

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

An In vitro Co-infection Model to Study Plasmodium falciparum-HIV-1 Interactions in Human Primary Monocyte-derived Immune Cells

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Read Article