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多重蛍光免疫組織化学染色、イメージング、およびリンパ腫の組織学的試料中の分析
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Immunology and Infection
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JoVE Journal Immunology and Infection
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
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多重蛍光免疫組織化学染色、イメージング、およびリンパ腫の組織学的試料中の分析

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07:52 min

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January 09, 2019

DOI:

10.3791/58711-v

DOI:
10.3791/58711-v

•

07:52 min
January 09, 2019

•

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, , , , , , , , ,
1Department of Laboratory Medicine,AnSteel Group General Hospital, 2Cancer Science Institute of Singapore,National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, 4Department of Haematology-Oncology,National University Health System

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Multiplexed Fluorescent ImmunohistochemistryLymphomaTumor MicroenvironmentAntigen Co-expressionSpatial RelationshipAntigen RetrievalMicrowave StrippingPeroxidase BlockTyramide-based Fluorescent ReagentSpectral Imaging

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Hong, G., Fan, S., Phyu, T., Maheshwari, P., Hoppe, M. M., Phuong, H. M., de Mel, S., Poon, M., Ng, S., Jeyasekharan, A. D. Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma. J. Vis. Exp. (143), e58711, doi:10.3791/58711 (2019).

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